How to read CPV test paper: comprehensive explanation of usage and result determination
Recently, discussions about pet health testing have become increasingly popular, with the use of CPV (canine parvovirus) test strips becoming the focus of pet owners. This article combines hot topics on the Internet in the past 10 days to provide you with a detailed analysis of the operating steps, result interpretation and precautions of CPV test strips, and attaches structured data for reference.
1. Working principle of CPV test paper
CPV test paper uses immunochromatography technology to quickly determine the infection status by detecting viral antigens in dog feces. The test paper contains a quality control line (C line) and a detection line (T line). When both lines are colored, it is a positive result.
| Test strip area | Function description | Color rendering significance |
|---|---|---|
| Sample area(S) | Add sample to be tested | Liquid penetration starting point |
| Quality control line (C) | Test strip validity verification | Must develop color |
| Detection line (T) | Viral Antigen Test | Color develops when positive |
2. Detailed explanation of operating steps
1.sample collection: Use the matching cotton swab to collect fresh dog feces (loose feces are preferred)
2.Sample processing: Dip a cotton swab into the diluent and stir for 10 seconds, then let it sit for 1 minute.
3.Add sample dropwise: Use a dropper to absorb the supernatant and drop 4-5 drops vertically into the sample hole of the test paper.
4.wait for reaction: Place the test strip flat and read the result within 10-15 minutes
| Time node | Observation points | Things to note |
|---|---|---|
| 0-5 minutes | liquid flow | Make sure the liquid soaks through the C line |
| 10 minutes | Initial color development | Avoid direct sunlight |
| 15 minutes later | final judgment | Timeout result is invalid |
3. Result Judgment Criteria
The results can be divided into three types according to the color development of the test strip:
positive result: Both the C line and T line are colored (even if the T line is lighter in color)
negative result: Only C line shows color, T line has no color
Invalid result: Line C does not develop color (need to replace the test paper and retest)
| result type | Color characteristics | clinical significance |
|---|---|---|
| Strong positive | Line C and T are all dark red | higher viral load |
| weak positive | T line is shallower than C line | Early infection may |
| false negative | Only C line shows color | Need to be combined with PCR review |
4. Answers to frequently asked questions (summary of hot topics)
1.Test paper accuracy:Clinical data shows that the sensitivity is about 85% and the specificity is about 92%. False negatives may occur in the early stage of the disease.
2.Detection time: It is recommended to test 24-48 hours after the onset of vomiting/diarrhea symptoms
3.Save requirements: The test paper needs to be stored dry and protected from light at 2-30℃ and used immediately after opening.
4.vaccine interference: Vaccination may cause false positive within 7 days
5. Things to note
• The environment needs to be thoroughly disinfected before and after testing to avoid cross-infection
• Positive results should immediately isolate the sick dog and seek medical attention
• Test strips cannot be used as the only basis for diagnosis and must be combined with clinical symptoms
• Do not mix different brands of test strips with diluent
Recent pet hospital data shows that the CPV positive detection rate increased by 12% from the previous month, reminding pet-raising families to pay attention to environmental disinfection and vaccination. Mastering the correct method of using test strips can help detect diseases early and gain golden time for treatment.
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